1WSG

Co-crystal structure of E.coli RNase HI active site mutant (E48A/D134N*) with Mn2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Detector technology	CCD
Collection date	2003-09-26
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	120.223, 79.879, 64.198
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.200
Rwork	0.221
R-free	0.29000
Structure solution method	MOLECULAR REPLACEMENT
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200	2.200
Number of reflections	31685
Completeness [%]	98.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1