1WSE

Co-crystal structure of E.coli RNase HI active site mutant (E48A*) with Mn2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Detector technology	CCD
Collection date	2004-02-16
Detector	MARRESEARCH
Wavelength(s)	0.9791
Spacegroup name	P 2 2 21
Unit cell lengths	58.041, 66.008, 79.485
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.300
Rwork	0.230
R-free	0.28800
Structure solution method	MOLECULAR REPLACEMENT
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300	2.300
Number of reflections	13962
Completeness [%]	98.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1