1WQ6
The tetramer structure of the nervy homolgy two (NHR2) domain of AML1-ETO is critical for AML1-ETO'S activity
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-05-29 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97946, 0.97915, 0.96422 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 60.720, 75.442, 30.781 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.000 |
| R-factor | 0.21575 |
| Rwork | 0.213 |
| R-free | 0.27012 |
| Structure solution method | MAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.794 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.044 | 0.196 |
| Number of reflections | 10096 | |
| <I/σ(I)> | 24.9 | 5.5 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 6.7 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 100mM Na citrate (pH 5.6), 100mM MgCl2, 30% MPD, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
