1WAX
Protein tyrosine phosphatase 1B with active site inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-25 |
| Detector | RIGAKU CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 88.095, 88.095, 104.497 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.050 - 2.200 |
| R-factor | 0.201 |
| Rwork | 0.198 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c83 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.424 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | IN-HOUSE (SOFTWARE) |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.080 | 0.340 |
| Number of reflections | 24030 | |
| <I/σ(I)> | 4.1 | 1.6 |
| Completeness [%] | 96.7 | 88.2 |
| Redundancy | 1.8 | 1.72 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | pH 7.50 |
