1W8P
Structural properties of the B25Tyr-NMe-B26Phe insulin mutant.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.903, 62.116, 47.796 |
| Unit cell angles | 90.00, 110.58, 90.00 |
Refinement procedure
| Resolution | 25.570 - 2.080 |
| R-factor | 0.192 |
| Rwork | 0.189 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1evr |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.708 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.560 | 2.190 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.030 | 0.120 |
| Number of reflections | 17496 | |
| <I/σ(I)> | 6 | |
| Completeness [%] | 88.0 | 89 |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.1 M TRI-SODIUM CITRATE, 0.02 % W/V ZINC ACETATE, 6 % W/V TRIS/HCL PH 8.2, 0.1 % W/V PHENOL. |
