1W55

Structure of the Bifunctional IspDF from Campylobacter jejuni

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	160
Detector technology	CCD
Collection date	2002-06-21
Detector	ADSC CCD
Spacegroup name	P 63 2 2
Unit cell lengths	108.758, 108.758, 161.262
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	14.960 - 2.300
R-factor	0.17
Rwork	0.167
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1I52 AND 1GX1
RMSD bond length	0.031
RMSD bond angle	1.744
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	14.960	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.070	0.360
Number of reflections	489834
<I/σ(I)>	41.5	5.3
Completeness [%]	98.4	87.2
Redundancy	19.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				35 % ETHYLENE GLYCOL