1W37
2-keto-3-deoxygluconate(KDG) aldolase of Sulfolobus solfataricus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-07-11 |
| Detector | R-AXIS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.577, 131.553, 132.556 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 95.350 - 2.000 |
| R-factor | 0.158 |
| Rwork | 0.153 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MAD PHASED STRUCTURE |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.730 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 | 0.160 |
| Number of reflections | 98295 | |
| <I/σ(I)> | 10.8 | 4.6 |
| Completeness [%] | 99.1 | 99.1 |
| Redundancy | 3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 0.1M HEPES PH6, 13%PEG4000, 8% ISOPROPANOL, pH 6.00 |
