1W1G
Crystal Structure of the PDK1 Pleckstrin Homology (PH) domain bound to DiC4-phosphatidylinositol (3,4,5)-trisphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-09-15 |
| Detector | RIGAKU RAXIS 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.264, 58.943, 36.440 |
| Unit cell angles | 90.00, 102.33, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.450 |
| R-factor | 0.183 |
| Rwork | 0.182 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDK1PH INSP4 MODEL |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.703 |
| Data reduction software | CrystalClear |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.050 | 0.370 |
| Number of reflections | 25810 | |
| <I/σ(I)> | 8.8 | 2.5 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 2.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.2 | 25 % PEG 4000, 0.1 M SODIUM ACETATE [PH 4.2], 0.3 M AMMONIUM ACETATE |
