1VYA
Identification and characterization of the first plant G-quadruplex binding protein encoded by the Zea mays L. nucleoside diphosphate1 gene, ZmNDPK1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 277 |
| Detector technology | CCD |
| Collection date | 2013-11-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.962, 179.198, 88.469 |
| Unit cell angles | 90.00, 99.28, 90.00 |
Refinement procedure
| Resolution | 39.859 - 2.050 |
| R-factor | 0.1609 |
| Rwork | 0.160 |
| R-free | 0.20110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u8w |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.013 |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.120 | 0.660 |
| Number of reflections | 123697 | |
| <I/σ(I)> | 19.8 | 1.75 |
| Completeness [%] | 96.3 | 92.3 |
| Redundancy | 7.3 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM sodium cacodylate (pH 6.5), 12% PEG 8000, and 200 mM CaCl2 |
