1VR3

Crystal structure of Acireductone dioxygenase (13543033) from Mus musculus at 2.06 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-10
Detector	ADSC
Wavelength(s)	0.979735,0.979562,0.956885
Spacegroup name	P 41 21 2
Unit cell lengths	79.136, 79.136, 114.128
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.530 - 2.060
R-factor	0.16387
Rwork	0.162
R-free	0.19532
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.436
Data reduction software	XDS
Data scaling software	XDS
Phasing software	autoSHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.540	2.170
High resolution limit [Å]	2.060	2.060
Number of reflections	23148
<I/σ(I)>	13.6	2.2
Completeness [%]	100.0	100
Redundancy	6.8	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	5.6	277	5.0% Glycerol, 19.0% iso-Propanol, 19.0% PEG-4000, 0.1M Citrate pH 5.6, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	5.6	277	5.0% Glycerol, 19.0% iso-Propanol, 19.0% PEG-4000, 0.1M Citrate pH 5.6, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K