1VPX
Crystal structure of Transaldolase (EC 2.2.1.2) (TM0295) from Thermotoga maritima at 2.40 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-17 |
Detector | ADSC |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 145.298, 104.419, 171.124 |
Unit cell angles | 90.00, 108.99, 90.00 |
Refinement procedure
Resolution | 83.130 - 2.400 |
R-factor | 0.19884 |
Rwork | 0.196 |
R-free | 0.24694 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1l6w |
RMSD bond length | 0.016 |
RMSD bond angle | 1.508 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (CCP4 4.2) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 104.190 | 2.460 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 162044 | |
<I/σ(I)> | 9.1 | 1.3 |
Completeness [%] | 85.9 | 56.1 |
Redundancy | 2.5 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 7 | 293 | 30.0% 1,2-propanediol, 0.2M (NH4)2SO4, 10.0% Glycerol, 0.1M HEPES, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K, pH 7.0 |