1VOL
TFIIB (HUMAN CORE DOMAIN)/TBP (A.THALIANA)/TATA ELEMENT TERNARY COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Temperature [K] | 123 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.900, 78.000, 134.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.700 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.31800 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.860 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.700 |
| Rmerge | 0.072 * |
| Total number of observations | 180515 * |
| Number of reflections | 20995 |
| Completeness [%] | 82.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8.5 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA complex | 0.3-0.4 (mM) | |
| 10 | 1 | reservoir | glycerol | 5-10 (%(v/v)) | |
| 11 | 1 | reservoir | dithiothreitol | 20 (mM) | |
| 12 | 1 | reservoir | Tris-HCl | 40 (mM) | |
| 13 | 1 | drop | ethylene glycerol | 2 (%(v/v)) | |
| 2 | 1 | drop | 40 (mM) | ||
| 3 | 1 | drop | ammonium acetate | 300 (mM) | |
| 4 | 1 | drop | 5 (mM) | ||
| 5 | 1 | drop | 5 (mM) | ||
| 6 | 1 | drop | zinc acetate | 0.01 (mM) | |
| 7 | 1 | drop | dithiothreitol | 10 (mM) | |
| 8 | 1 | drop | glycerol | 10 (%(v/v)) | |
| 9 | 1 | drop | Tris-HCl | 40 (mM) |
