1VLK

STRUCTURE OF VIRAL INTERLEUKIN-10

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1996-05-04
Detector	RIGAKU
Spacegroup name	P 43 21 2
Unit cell lengths	36.270, 36.270, 219.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.900
R-factor	0.191 *
Rwork	0.191
RMSD bond length	0.014
RMSD bond angle	0.052
Data reduction software	DENZO
Refinement software	PROFFT

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	1.900 *	1.900 *
Rmerge	0.081
Number of reflections	9860
Completeness [%]	82.0	47 *
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop	PEG4000	3.4 (mg/ml)
3	1	drop	sodium acetate	0.02 (M)
4	1	drop	ammonium sulfate	0.04 (M)
5	1	reservoir	PEG4000	14 (%)
6	1	reservoir	sodium acetate	0.1 (M)
7	1	reservoir	ammonium sulfate	0.2 (M)