1VKP
X-RAY STRUCTURE OF GENE PRODUCT FROM ARABIDOPSIS THALIANA AT5G08170, AGMATINE IMINOHYDROLASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 32-ID |
Synchrotron site | APS |
Beamline | 32-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-04-04 |
Detector | MAR CCD 165 mm |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.020, 115.813, 66.634 |
Unit cell angles | 90.00, 97.10, 90.00 |
Refinement procedure
Resolution | 19.880 - 1.530 |
R-factor | 0.148 |
Rwork | 0.146 |
R-free | 0.17400 |
Structure solution method | SAD |
RMSD bond length | 0.020 |
RMSD bond angle | 1.556 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.06) |
Refinement software | REFMAC (refmac_5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.580 |
High resolution limit [Å] | 1.530 | 1.530 |
Rmerge | 0.091 | 0.322 |
Number of reflections | 127993 | |
<I/σ(I)> | 11.69 | 3.23 |
Completeness [%] | 98.2 | 96.3 |
Redundancy | 7.9 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 297 | 10 mg/ml protein, 18% PEG 4000, 8% ethylene glycol, 100 mM MOPS, Vapor diffusion, hanging drop, pH 7.00, temperature 297K |