1VK2

Crystal structure of Uracil-DNA glycosylase (TM0511) from Thermotoga maritima at 1.90 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-03-03
Detector	ADSC
Wavelength(s)	1.0000, 0.9796, 0.9794
Spacegroup name	P 43 21 2
Unit cell lengths	61.049, 61.049, 127.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.090 - 1.900
R-factor	0.17886
Rwork	0.177
R-free	0.20573
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.671
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.900	1.900
Number of reflections	19816
<I/σ(I)>	25.15	2.03
Completeness [%]	100.0	99.95
Redundancy	7.87	6.13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	8.5	277	TRIS pH 8.5, 8% PEG-8000, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K. cryo condition: 25% PEG-200.