1VIN
BOVINE CYCLIN A3
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.700, 60.900, 83.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.000 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.29900 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.410 * |
| Data reduction software | DENZO |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.000 * |
| Rmerge | 0.068 |
| Number of reflections | 17065 |
| Completeness [%] | 91.4 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8.5 * | 20 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | cyclin A-3 | 5-10 (mg/ml) | |
| 2 | 1 | drop | 0.1 (M) | ||
| 3 | 1 | drop | monothioglycerol | 0.01 (%) | |
| 4 | 1 | drop | 0.01 (%) | ||
| 5 | 1 | drop | Tris-HCl | 50 (mM) | |
| 6 | 1 | drop | reservoir | ||
| 7 | 1 | reservoir | methoxyPEG5000 | 25-26 (%) | |
| 8 | 1 | reservoir | 0.2 (M) | ||
| 9 | 1 | reservoir | Tris-HCl | 0.1 (M) |
