1VHH
A POTENTIAL CATALYTIC SITE WITHIN THE AMINO-TERMINAL SIGNALLING DOMAIN OF SONIC HEDGEHOG
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-01-06 |
| Detector | FUJI |
| Wavelength(s) | 0.9686, 0.9792, 0.9793, 0.9879 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.700, 79.000, 35.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.700 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.26100 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 22.420 * |
| Data reduction software | DENZO |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.700 |
| Rmerge | 0.080 |
| Number of reflections | 14968 |
| Completeness [%] | 86.4 |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3.4 (mg/ml) | |
| 2 | 1 | drop | beta-mercaptoethanol | 1.4 (mM) | |
| 3 | 1 | reservoir | PEG8000 | 30 (%) | |
| 4 | 1 | reservoir | ammonium sulfate | 0.12 (M) | |
| 5 | 1 | reservoir | sodium cacodylate | 10 (mM) |
