1VGA
Structures of unligated and inhibitor complexes of W168F mutant of Triosephosphate Isomerase from Plasmodium falciparum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.730, 106.157, 89.526 |
| Unit cell angles | 90.00, 92.42, 90.00 |
Refinement procedure
| Resolution | 17.200 - 1.800 |
| Rwork | 0.207 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ydv |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.210 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.036 | 0.214 |
| Completeness [%] | 89 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291 | PEG1450, Lithium sulfate and Soduim acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
