1VEV
Crystal structure of peptide deformylase from Leptospira Interrogans (LiPDF) at pH6.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BSRF BEAMLINE 3W1A |
Synchrotron site | BSRF |
Beamline | 3W1A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-03-01 |
Detector | MARRESEARCH |
Wavelength(s) | 1.20 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 82.192, 82.192, 201.040 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.910 - 2.510 |
R-factor | 0.258 |
Rwork | 0.258 |
R-free | 0.29000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB id 1SV2 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.384 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.910 | 2.660 |
High resolution limit [Å] | 2.510 | 2.500 |
Number of reflections | 21663 | |
Completeness [%] | 87.7 | 75.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 4M formate sodium, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |