1VCV
Structure of 2-deoxyribose-5-phosphate aldolase from Pyrobaculum aerophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-06 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 63 |
| Unit cell lengths | 84.540, 84.540, 112.620 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.070 - 2.000 |
| R-factor | 0.2095 |
| Rwork | 0.206 |
| R-free | 0.24100 |
| Structure solution method | MIRAS |
| Data reduction software | CrystalClear |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 28.070 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 28389 |
| Completeness [%] | 100.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 20% PEG1000, 0.1M ACETATE BUFFER, 0.2M ZINC ACETATE, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
