1UZN
MabA from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-08-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.539, 117.111, 51.869 |
| Unit cell angles | 90.00, 122.62, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.910 |
| R-factor | 0.187 |
| Rwork | 0.185 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.182 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.960 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.052 | 0.150 |
| Number of reflections | 28001 | |
| <I/σ(I)> | 17 | 4.3 |
| Completeness [%] | 93.1 | 76.2 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.6 | PIPES PH 6.6 CSCL 400 MM, PEG 3000 12%, NADP 100 MM |
