1UX6

Structure of a thrombospondin C-terminal fragment reveals a novel calcium core in the type 3 repeats

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Collection date	2004-09-15
Detector	ADSC CCD
Spacegroup name	H 3 2
Unit cell lengths	162.340, 162.340, 85.140
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.900
R-factor	0.199
Rwork	0.199
R-free	0.22700
Structure solution method	MIRAS
RMSD bond length	0.005
RMSD bond angle	1.400
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MLPHARE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.086	0.370
Number of reflections	32099
Completeness [%]	95.3	92.9
Redundancy	5.4	4.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		pH 7.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6-10 (mg/ml)
2	1	reservoir	MPD	60 (%)
3	1	reservoir	HEPES	100 (mM)	pH7.5
4	1	reservoir		5 (mM)