1USY
ATP phosphoribosyl transferase (HisG:HisZ) complex from Thermotoga maritima
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-06-15 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 101.580, 134.403, 154.206 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 12.000 - 2.520 |
R-factor | 0.207 |
Rwork | 0.203 |
R-free | 0.28700 |
Structure solution method | MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.798 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.055 | 0.289 |
Number of reflections | 52743 | |
<I/σ(I)> | 18.44 | 6.86 |
Completeness [%] | 98.4 | 98.1 |
Redundancy | 17.6 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.2 | pH 4.20 |