1UQU

Trehalose-6-phosphate from E. coli bound with UDP-glucose.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Wavelength(s)	0.933
Spacegroup name	P 21 21 2
Unit cell lengths	88.905, 102.310, 118.924
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.000
R-factor	0.234
Rwork	0.232
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gz5
RMSD bond length	0.014
RMSD bond angle	1.490
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.084	0.449
Number of reflections	69411
<I/σ(I)>	12.4	1.79
Completeness [%]	94.0	68
Redundancy	3.10 *	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8		30% PEG 4000, 200MM AMMONIUM ACETATE, 100MM TRISHCL PH 8

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	20 (mM)	pH8.
2	1	drop		200 (mM)
3	1	drop	protein	10 (mg/ml)