1UPS
GlcNAc[alpha]1-4Gal releasing endo-[beta]-galactosidase from Clostridium perfringens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 63 |
| Unit cell lengths | 160.430, 160.430, 86.140 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.820 |
| R-factor | 0.177 |
| Rwork | 0.176 |
| R-free | 0.20400 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.219 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE/RESOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.058 | 0.218 |
| Number of reflections | 107796 | |
| <I/σ(I)> | 25.1 | 8.1 |
| Completeness [%] | 95.8 | 94.4 |
| Redundancy | 5.8 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 42% AMMONIUM SULFATE, 2% PEG400, 0.1M HEPES-NA AT PH 7.4 |
