1UDU
Crystal structure of Human Phosphodiesterase 5 complexed with tadalafil(Cialis)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6B |
| Synchrotron site | PAL/PLS |
| Beamline | 6B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-04-22 |
| Detector | MACSCIENCE |
| Wavelength(s) | 1.12714 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 131.352, 48.561, 123.863 |
| Unit cell angles | 90.00, 117.28, 90.00 |
Refinement procedure
| Resolution | 19.590 - 2.830 |
| R-factor | 0.263 |
| Rwork | 0.263 |
| R-free | 0.37400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.130 | 2.900 |
| High resolution limit [Å] | 2.830 | 2.800 |
| Number of reflections | 12117 | |
| Completeness [%] | 79.7 | 74.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | PEG8000, Tris buffer, Magnesium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
