1UCB

STRUCTURE OF UNCOMPLEXED FAB COMPARED TO COMPLEX (1CLY, 1CLZ)

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	295
Detector technology	AREA DETECTOR
Collection date	1993-03
Detector	SIEMENS-NICOLET X100
Spacegroup name	P 21 21 2
Unit cell lengths	61.100, 174.300, 45.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.500
R-factor	0.2
Rwork	0.200
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	FV OF 1CLZ WITH CDRS REMOVED 1CLY (HUMAN IGG1 HUMAN KAPPA) AND OF 1CLZ (MOUSE IGG3 MOUSE KAPPA)
RMSD bond length	0.009
RMSD bond angle	28.000 *
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MERLOT
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.000	2.800
High resolution limit [Å]	2.500	2.530 *
Rmerge	0.045	0.107
Total number of observations	29571 *
Number of reflections	14826
<I/σ(I)>	16.1	4
Completeness [%]	87.0	65
Redundancy	2	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7 *		0.1 M SODIUM ACETATE (PH 4.6), 0.1 M AMMONIUM SULFATE, 15 % PEG 8000.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	8 (mg/ml)
2	1	2	imidazole-HCl	10 (mM)