1UC3
Crystal Structure of hemoglobin I from river lamprey
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 100 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1999-06-08 |
| Detector | WEISSENBERG |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.210, 73.610, 105.870 |
| Unit cell angles | 103.95, 91.52, 97.64 |
Refinement procedure
| Resolution | 10.000 - 2.300 |
| Rwork | 0.197 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lhb |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.141 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.072 | 0.255 |
| Number of reflections | 76404 | |
| <I/σ(I)> | 14.6 | 3.4 |
| Completeness [%] | 97.8 | 94.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 283 | 0.1M HEPES, 21% PEG6000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
