1U7W

Phosphopantothenoylcysteine synthetase from E. coli, CTP-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Detector technology	CCD
Collection date	2003-03-04
Detector	MARRESEARCH
Wavelength(s)	0.9789
Spacegroup name	C 2 2 21
Unit cell lengths	45.926, 144.406, 246.215
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.225
Rwork	0.211
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u7u
RMSD bond length	0.011
RMSD bond angle	1.620
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.500
Rmerge	0.065
Number of reflections	28789
Completeness [%]	99.5
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.6	293	PEG 3000, pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K