1U7U
Phosphopantothenoylcysteine synthetase from E. coli
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.95 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 52.930, 57.255, 78.756 |
| Unit cell angles | 90.00, 108.86, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.236 |
| Rwork | 0.221 |
| R-free | 0.27900 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.730 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.054 |
| Number of reflections | 8804 |
| Completeness [%] | 99.9 |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | PEG 3350, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
