1U6J

The Structure of native coenzyme F420-dependent methylenetetrahydromethanopterin dehydrogenase at 2.4A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2002-11-19
Detector	MARRESEARCH
Wavelength(s)	1.05
Spacegroup name	C 1 2 1
Unit cell lengths	150.900, 118.900, 219.200
Unit cell angles	90.00, 90.01, 90.00

Refinement procedure

Resolution	20.000 - 2.400
Rwork	0.175
R-free	0.23500
Starting model (for MR)	Selenomethionine-labelled METHYLENETETRAHYDROMETHANOPTERIN DEHYDROGENASE (MTD) PDB-ID 1QV9
RMSD bond length	0.007
RMSD bond angle	1.250
Data scaling software	XDS
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.550
High resolution limit [Å]	2.400	2.400
Rmerge	0.108	0.302
Number of reflections	144106
<I/σ(I)>	14.2	5.9
Completeness [%]	95.0	88.6
Redundancy	4.8	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	MOPS, KOH, MPD, PEG 400, Mg acetate, Na phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K