1U54
Crystal Structures of the Phosphorylated and Unphosphorylated Kinase Domains of the CDC42-associated Tyrosine Kinase ACK1 bound to AMP-PCP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-01-21 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 200.384, 42.332, 70.848 |
| Unit cell angles | 90.00, 96.28, 90.00 |
Refinement procedure
| Resolution | 38.710 - 2.800 |
| R-factor | 0.226 |
| Rwork | 0.222 |
| R-free | 0.32200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u46 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.972 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.710 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 14697 | |
| <I/σ(I)> | 12.9 | 3.7 |
| Completeness [%] | 98.3 | 97.8 |
| Redundancy | 3.6 | 3.64 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 291 | PEG 4000, lithium sulfate, magnesium chloride, sodium chloride, AMP-PCP, Tris, TCEP, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K, pH 8.50 |
