1U0U
An Aldol Switch Discovered in Stilbene Synthases Mediates Cyclization Specificity of Type III Polyketide Synthases: Pine stilbene synthase structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-04-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.221, 361.291, 57.317 |
| Unit cell angles | 90.00, 98.39, 90.00 |
Refinement procedure
| Resolution | 54.100 - 2.110 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bi5 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data scaling software | PROW |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.100 | 2.230 |
| High resolution limit [Å] | 2.110 | 2.100 |
| Number of reflections | 103258 | |
| Completeness [%] | 78.7 | 38.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | PEG 8000, ammonium acetate, MOPSO-Na+, DTT, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
