1TZO
Crystal Structure of the Anthrax Toxin Protective Antigen Heptameric Prepore
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-04-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0800 |
| Spacegroup name | P 1 |
| Unit cell lengths | 149.608, 167.028, 168.200 |
| Unit cell angles | 77.56, 75.74, 76.01 |
Refinement procedure
| Resolution | 30.000 - 3.600 |
| R-factor | 0.315 |
| Rwork | 0.314 |
| R-free | 0.31700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4.5 PA heptamer model |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.097 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.730 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Number of reflections | 163256 | |
| <I/σ(I)> | 10.6 | 1.6 |
| Completeness [%] | 93.4 | 68.8 |
| Redundancy | 2 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.25 | 298 | HEPES, sodium chloride, b-octylglucoside, spermine, pH 8.25, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
