1TYG

Structure of the thiazole synthase/ThiS complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 8-BM
Synchrotron site	APS
Beamline	8-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2003-07-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	P 65 2 2
Unit cell lengths	91.654, 91.654, 401.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 3.150
R-factor	0.24691
Rwork	0.242
R-free	0.30460
Structure solution method	SAD
RMSD bond length	0.022
RMSD bond angle	2.022
Data reduction software	MOSFLM
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	3.260
High resolution limit [Å]	3.150	3.150
Number of reflections	17866
Completeness [%]	98.2	99.8
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.15	293	5% PEG 8K, 200 mM Sodium Chloride, 100 mM sodium phosphate pH 6.1, pH 7.15, VAPOR DIFFUSION, HANGING DROP, temperature 293K