1TXF
CRYSTAL STRUCTURE OF THE GLUR5 LIGAND BINDING CORE IN COMPLEX WITH GLUTAMATE AT 2.1 ANGSTROM RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.99997 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.809, 63.710, 50.354 |
| Unit cell angles | 90.00, 107.22, 90.00 |
Refinement procedure
| Resolution | 32.400 - 2.100 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s7y |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.045 | 0.269 |
| Number of reflections | 21345 | |
| <I/σ(I)> | 12.7 | 2.2 |
| Completeness [%] | 84.3 | 49.7 |
| Redundancy | 2.5 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% PEG 10K, 100 mM HEPES, 20 mM NaCl, 1 mM EDTA, 10 mM Glutamate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
