1TVH
Crystal structure of Modified Melanoma Antigen gp100(209-T2M) Bound to Human Class I MHC HLA-A2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-21 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.98731 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.407, 84.485, 84.014 |
| Unit cell angles | 90.00, 90.11, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.802 |
| R-factor | 0.18795 |
| Rwork | 0.185 |
| R-free | 0.24651 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bor |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.140 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.065 | 0.400 |
| Number of reflections | 74168 | |
| <I/σ(I)> | 16.3 | 2.72 |
| Completeness [%] | 98.7 | 97 |
| Redundancy | 2.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | PEG 3350, MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
