1TVE
Homoserine Dehydrogenase in complex with 4-(4-hydroxy-3-isopropylphenylthio)-2-isopropylphenol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0722 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.456, 54.379, 92.497 |
| Unit cell angles | 90.00, 98.97, 90.00 |
Refinement procedure
| Resolution | 35.240 - 3.000 |
| R-factor | 0.289 |
| Rwork | 0.289 |
| R-free | 0.36300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ebu |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.240 |
| High resolution limit [Å] | 3.000 |
| Rmerge | 0.085 |
| Number of reflections | 14617 |
| <I/σ(I)> | 7.3 |
| Completeness [%] | 99.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 295 | Ches, PEG 600, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
