1TT9
Structure of the bifunctional and Golgi associated formiminotransferase cyclodeaminase octamer
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 150 |
Detector technology | CCD |
Collection date | 2003-11-04 |
Detector | SBC-2 |
Wavelength(s) | 0.9791, 0.9793, 0.9716 |
Spacegroup name | P 4 |
Unit cell lengths | 134.848, 134.848, 156.365 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 3.420 |
R-factor | 0.251 |
Rwork | 0.251 |
R-free | 0.29300 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.600 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.600 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.060 | 0.249 |
Number of reflections | 31393 | |
<I/σ(I)> | 6 | 1.9 |
Completeness [%] | 86.6 | 64.7 |
Redundancy | 8 | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 298 | PEG 600, sodium sulphate, HEPES, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |