1TQC

Ovine recombinant PrP(114-234), ARR variant in complex with the VRQ14 Fab fragment (IgG2a)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Detector technology	CCD
Collection date	2003-08-22
Detector	MARRESEARCH
Wavelength(s)	0.97926
Spacegroup name	P 21 21 2
Unit cell lengths	91.659, 145.448, 43.596
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.950 - 2.800
R-factor	0.222
Rwork	0.222
R-free	0.28800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.870
High resolution limit [Å]	2.800	2.800
Rmerge	0.087	0.375
Number of reflections	15206
<I/σ(I)>	17.1	4.2
Completeness [%]	99.5	99.8
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	291	Sodium citrate 0.1M , PEG8000, Ammonium acetate 0.2M, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K