1TH1
Beta-catenin in complex with a phosphorylated APC 20aa repeat fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2004-05-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97970 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.159, 133.800, 82.166 |
| Unit cell angles | 90.00, 110.85, 90.00 |
Refinement procedure
| Resolution | 47.980 - 2.500 |
| Rwork | 0.225 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qz7 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.630 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.095 | 0.795 |
| Number of reflections | 59245 | |
| <I/σ(I)> | 24.6 | 2.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.4 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Tris, sidium chloride, PEG2000MME, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
