1TFC

CRYSTAL STRUCTURE OF THE LIGAND-BINDING DOMAIN OF THE ESTROGEN-RELATED RECEPTOR GAMMA IN COMPLEX WITH A STEROID RECEPTOR COACTIVATOR-1 PEPTIDE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2003-04-13
Detector	ADSC QUANTUM 4
Wavelength(s)	0.933
Spacegroup name	P 43 21 2
Unit cell lengths	83.477, 83.477, 239.401
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.320 - 2.400
R-factor	0.243
Rwork	0.243
R-free	0.25800
Structure solution method	1KV6 refined to higher resolution
Starting model (for MR)	PBD ENTRY 1KV6
RMSD bond length	0.007
RMSD bond angle	1.130
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	34152
<I/σ(I)>	54.9
Completeness [%]	99.9	94.8
Redundancy	10.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	TRIS.HCL, AMMONIUM SULFATE, GLYCEROL, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K