1T7P

T7 DNA POLYMERASE COMPLEXED TO DNA PRIMER/TEMPLATE,A NUCLEOSIDE TRIPHOSPHATE, AND ITS PROCESSIVITY FACTOR THIOREDOXIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-11
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	106.600, 216.300, 52.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.200
R-factor	0.24
Rwork	0.240
R-free	0.27900
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	22.860 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.054 *	0.151 *
Total number of observations	162012 *
Number of reflections	60190 *
<I/σ(I)>	20.8	6.5
Completeness [%]	96.5	86.7
Redundancy	2.7	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5 *		used to seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG8000	12 (%(w/v))
2	1	1	ACES	100 (mM)
3	1	1	ammonium sulfate	120 (mM)
4	1	1		30 (mM)
5	1	1	dithiothreitol	5 (mM)