1T6S

Crystal structure of a conserved hypothetical protein from Chlorobium tepidum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-25
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0000
Spacegroup name	P 62
Unit cell lengths	69.420, 69.420, 117.990
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.950
R-factor	0.23
Rwork	0.216
R-free	0.25310
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.240
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	500.000	1.980
High resolution limit [Å]	1.950	1.950
Number of reflections	23532
<I/σ(I)>	11.6
Completeness [%]	99.3	95.7
Redundancy	5.67

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	ammonium nitrate, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K