1T6B
Crystal structure of B. anthracis Protective Antigen complexed with human Anthrax toxin receptor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2004-04-11 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.892 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 88.160, 94.118, 135.597 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.500 |
R-factor | 0.21009 |
Rwork | 0.207 |
R-free | 0.26572 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
RMSD bond angle | 1.649 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 39917 | |
<I/σ(I)> | 11.5 | 2.4 |
Completeness [%] | 99.9 | |
Redundancy | 5.3 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 300 | CHES buffer, PEG400, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 300K |