1T65

Crystal structure of the androgen receptor ligand binding domain with DHT and a peptide derived form its physiological coactivator GRIP1 NR box 2 bound in a non-helical conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-07-02
Detector	ADSC QUANTUM 4
Wavelength(s)	1.1
Spacegroup name	P 21 21 21
Unit cell lengths	55.600, 67.580, 69.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.200 - 1.660
R-factor	0.252
Rwork	0.230
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond angle	18.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS (1.1)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	24.200
High resolution limit [Å]	1.660
Number of reflections	29066
Completeness [%]	92.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	hepes, Na-citrate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K