1T61
crystal structure of collagen IV NC1 domain from placenta basement membrane
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97471 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.650, 80.139, 235.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.500 |
| R-factor | 0.177 |
| Rwork | 0.174 |
| R-free | 0.20000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.350 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 215169 | |
| <I/σ(I)> | 12.4 | |
| Completeness [%] | 96.0 | 85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | PEG 20K, dioxane, tris buffer, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
