1T5Z

Crystal Structure of the Androgen Receptor Ligand Binding Domain (LBD) with DHT and a peptide derived from its physiological coactivator ARA70

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-07-02
Detector	ADSC QUANTUM 4
Wavelength(s)	1.1
Spacegroup name	P 21 21 21
Unit cell lengths	55.680, 66.423, 68.253
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.000 - 2.300
Rwork	0.228
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond angle	0.770
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.000
High resolution limit [Å]	2.300	2.300
Number of reflections	10881
<I/σ(I)>	5.1
Completeness [%]	82.5	90
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	Hepes, Na-citrate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K