1T3L

Structural Analysis of the Voltage-Dependent Calcium Channel Beta Subunit Functional Core in Complex with Alpha1 Interaction Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2003-07-07
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0069
Spacegroup name	P 21 21 2
Unit cell lengths	72.775, 168.262, 34.233
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	84.520 - 2.200
R-factor	0.22554
Rwork	0.221
R-free	0.28201
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.620
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200	2.200
Rmerge	0.059	0.310
Number of reflections	19123
<I/σ(I)>	21.3	3.9
Completeness [%]		63.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	PEG 20K, Bicine, sodium chloride, beta-mercaptoethanol, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 293K