1T2K
Structure Of The DNA Binding Domains Of IRF3, ATF-2 and Jun Bound To DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-07-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 186.380, 65.210, 83.850 |
| Unit cell angles | 90.00, 93.44, 90.00 |
Refinement procedure
| Resolution | 29.980 - 3.000 |
| R-factor | 0.257 |
| Rwork | 0.257 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2IRF and 2DGC |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.190 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 20151 | |
| <I/σ(I)> | 19.4 | 5.7 |
| Completeness [%] | 94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 100 mM NaCacodylate, pH 6.5, 12.5% (w/v) PEG 6000, 400 mM NH4OAc, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | NaCacodylate | ||
| 2 | 1 | 1 | PEG 6000 | ||
| 3 | 1 | 1 | NH4OAc | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | PEG 6000 | ||
| 6 | 1 | 2 | NaCacodylate | ||
| 7 | 1 | 2 | H2O |
